octobre 2013

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03-07-10 Adding details to describe SME Gal4VP16 clone . Derived clones coding sequence Reinhard Koester described in Koester et al. 2001 VP16 VP16 fragment is actually [ 413-470 ] , a transactivation domain of VP16 has been partly cut away , which reduces the toxicity of the fusion protein.
10-12-09 Added description of clone 550 ( version exactly SME mCherryCAAX ) . The previous version of this clone , No. 450 , has been contaminated by the H80D mutation in some preparations.
28/04/09 Minor to page list clones , clones pDONR changes all listed as intermediate clones. Has tilt to Yuk Fai Leung and Jim Lister.
18:12:07 Added information about the use of monkeys to predict sequences of expression clones .
18/12/07 Add Genbank format for all clones distributed .
12-09-07 Minor changes to the page att list to fix a few sequences and the names (especially sequences attBXX_primer ) .
11/05/07 mcherry sequence Added a page with instructions and assembly sequences predicted sequence for expression clones.
02/11/07 small update ( v1.2 ) by clones mCherryCAAX (no change) , EGFP ( no stop) and mCherry ( no stop) .
10:11:07 Adding an mcherry sequence archived manual Invitrogen, because the previous direct connection broke , and because its nomenclature seems unpredictable change copy.
23:09:07 Adding Sources page . The Wiki is now almost completed and entered a maintenance phase. We will continue to correct errors and information on new clones like the kit works .
9:12:07 Add a prepublication version of the document on Tol2kit methods , various other minor changes.
17:08:07 Wiki URL: a problem that some users have had to solve wiki with the firewall , we have changed something to access the site . You now have a slash at the end of the URL : http://chien.neuro.utah.edu/tol2kitwiki/
07.06.07 List of clones columns mcherry sequence for version numbers and the MTA have been added.
06/07/07 clone sequences : six bases (a KpnI ) lacked the predicted sequences for pDestTol2pA , pDestTol2pA2 , pDestTol2CG and pDestTol2CG2 . Maps and sequences were all corrected.
05:06:07 The results of the samples: mcherry sequence The pictures of the results of the sample taken with the laboratory building .
Links 5/11/07 : Note mcherry sequence that Invitrogen has released a new version of the manual multi-site gateway , 07:03:07 D version , which includes the use of the new enzyme LR clonase II Plus .
5/1/07 Home : Moved here from historical mcherry sequence revisions to the list page clone.
Sidebar 18:04:07 : Added links for quick navigation .
17:04:07 List Clone: sequences and pDestTol2pA pDestTol2CG (destination vector obsolete) provided .
03.23.07 List Clone : sequences and maps can now mcherry sequence be sent to all buildings .